Why is it important to follow lab safety rules

Biosafety rules for biology Labs

When we are working in a lab and dealing with medical equipment, heavy-duty supplies, and hazardous chemicals, the most important concern is lab safety. It is very important to consider proper usage and care of medical supplies when working in a lab because accidents are most likely to occur. This is a reason why is it important to follow lab safety rules

Why is it important to follow lab safety rules

Biosafety rules for biology labs are as follows;

  • We don’t have to do pipetting by mouth.
  • For containment, we must use cabinets of biological safety whenever there is a chance of generating droplets or aerosols during the experiment.
  • It’s good to practice carefully work with infectious fluids in order to avoid the generation of aerosols.
  • The lab technician when operating with cells, tissues, human blood, and body fluids must have to apply and understand universal safety precautions.
  • Where there are no alternatives, they have to restrict the usage of syringes and needles. To avoid self-inoculation, the lab technician must carefully use sharps, syringes, and needles.
  • Recapping of needles and syringes should be avoided.
  • The sharps have to dispose of in a correctly labeled and punctured resistant leak or container.
  • The person who is working in a lab and dealing with lab equipment should have to wear a lab coat, surgical gloves, and eyewear if needed.
  • They don’t have to wear the same lab coat outside of the laboratory as there is a chance of spreading contaminants.
  • After doing all lab activities, they have to wash hands, and properly removing PPE, and gloves in order to avoid infectious agents.
  • It is prohibited to eat, drink, smoke, apply cosmetics, and manipulate contact lenses. Storing of foods is not allowed in lab freezers and refrigerators. It is also not permitted to cook any food in the lab microwave Owen. (why it is important to follow lab safety rules)

 

What are Biological safety levels?

BSL (Biosafety levels) are sequences of protection referred to autoclave relevant activities that go on in specific biological labs. These are considered individual safeguards that are intended for lab personnel along with the community and the surrounding environment. (why it is important to follow lab safety rules)

Why is it important to follow lab safety rules

Biosafety level 1 (BSL1):

  • Biosafety level 1 is the lowest level among all biological safety levels.
  • The labs of Biosafety level 1 are used to work with agents that are not infectious and are not known for causing diseases to healthy animals and humans.
  • Here, standard practices of microbiology are followed and research is usually performed on standard open lab benches using special containment equipment.
  • These labs must have a sink available for washing hands and lab personnel should have to wear gloves and lab coats.
  • Several types of diseases are studied under BSL 1. These include canine hepatitis, non-pathogenic Escherichia coli, etc.

Biosafety level 2 (BSL2):

  • Biosafety level 2 is the second among biological safety levels.
  • The labs of biosafety level 2 are used to work with agents that are related to moderate human illnesses or infectious & pathogenic organisms.
  • Like BSL 1, standard practices of microbiology are followed along with enhanced measures.
  • The lab personnel must have to take greater care in order to protect themselves from injuries for example skin breaches and cuts along with mucous membrane exposure and ingestion.
  • The diseases which are usually studied under BSL 2 are staph infections, human immunodeficiency virus, and equine encephalitis viruses.

Biosafety level 3 (BSL3):

  • Biosafety level 3 is the third among biological safety levels.
  • The labs of biosafety level 3 are used to work with agents that are harmful/toxic or indigenous and are known for causing great harm to patients.
  • The microbes that are operated in these labs are known for causing potentially lethal diseases through the inhalation route.
  • The lab personnel could receive immunization against microbes they are working with.
  • These labs are often sternly registered and controlled by proper government agencies.
  • The diseases that are studied under BS-3 include the West Nile virus, tuberculosis, and yellow fever.

Biosafety level 3 (BSL4):

  • Biosafety level 4 is the 4th among biological safety levels.
  • The labs of biosafety level 4 are used to work with agents that are most harmful/toxic or indigenous and are known for causing great harm to patients.
  • The microbes that are operated in these labs are known for causing potentially most lethal diseases.
  • The lab personnel could receive immunization against microbes they are working with.
  • These labs are often sternly registered and controlled by proper government agencies.
  • The diseases that are studied under BS-4 include the Ebola virus.

Sample collection procedure for bacteria and human blood:

Sample collection procedure for human blood:

During the blood collection procedure, the safety of the phlebotomist and the patient must consider. The main purpose is to take the best likely sample for the performance of the test. Only licensed and trained personnel could obtain blood.

Why is it important to follow lab safety rules

The procedure of blood sample collection:

  • Firstly, it is imperative to make the patient comfortable whether he is reclining or sitting.
  • Throughout the whole procedure, while obtaining a blood sample, the personnel should wear gloves.
  • The next step is to tightly fasten a tourniquet around the arm of the patient, just directly above the elbow then instruct the patient to keep his/her arm straight and make a fist.
  • Then search the suitable vein by gently palpating the antecubital area. It is required to examine other wrists, hands, or arms for the best suitable vein.
  • When the vein has been found, clean carefully the site with alcohol swap and let it dry.
  • Then remove the needle cap of a syringe and grasp the hand of the patient with freehand of yours to hold underlying tissue taut and skin.
  • By rapid deliberate motion, insert the needle into the vein and start withdrawing the blood by pulling it back through the syringe plunger.
  • If blood doesn’t come, it is required to make tiny adjustments to the position of the needle for penetration of the vein.
  • When enough blood is withdrawn from the vein, release the tourniquet.
  • After successful procuration of blood, apply an alcohol swab to an area with pressure until bleeding stops.
  • Tourniquets and syringes should discard quickly after use.

Sample collection procedure for bacteria:

  • For dealing and accumulating with all specimens, use universal precautions.
  • All the specimens must be labeled appropriately with a patient identifier.
  • To collect bacteria on the end of the cotton swab, the first step is to roll a clean swab of cotton in the patient’s mouth. And then draw lightly a scribble with it on gelled agar.
  • You can also collect bacteria from any of the surfaces you want.
  • For getting a good sample, lightly dampen the swab of cotton with water. And then roll it in your finger so it easily touches all surfaces to be tested.
  • After collecting the bacterial sample, supply it in the laboratory as soon as possible for further examination.

See also what does hematology used for?

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